Re: AW: [Histonet] Chemicals that inactivate the primary antibody

[TF]

But the heat also damage the fluorescnece...
u need specific amplication kit anyway.


2009-05-17 



TF 



发件人： Gudrun Lang 
发送时间： 2009-05-17  02:00:18 
收件人： tifei <@t> foxmail.com 
抄送： histonet <@t> lists.utsouthwestern.edu 
主题： AW: [Histonet] Chemicals that inactivate the primary antibody 
 
For doublestaining the primary undergoes denaturation through a second
HIER-step. The second secondary ab doesn't bind to the first primary.
Therefore the binding sites must have been destroyed by heat in
retrieval-buffer.
Gudrun
-----Urspr黱gliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von TF
Gesendet: Samstag, 16. Mai 2009 18:48
An: Histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Chemicals that inactivate the primary antibody
Hi all, just wonder what kind of treatments/chemicals can complete block the
binding of 2nd antibody to binded primary antibody on antigen?
I tried HCl , but does not damage all the primaryantibody binding sites - i
can still see staining pattern finally.
2009-05-17 
TF 
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