[Histonet] Paraffin processing schedule-Cynamologus monkey tibia

[Roger Moretz]

Your schedule should be fine for any large tissue, and actually seems a bit overlong for mouse.  But if it works, it works.

Roger Moretz, ret.



----- Original Message ----
From: Danielle Crippen <dcrippen <@t> buckinstitute.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Friday, May 8, 2009 4:41:44 PM
Subject: [Histonet] Paraffin processing schedule-Cynamologus monkey tibia

Dear Histo-experts, 

Can you share your processing schedules for monkey (or human) bone
samples.  I need to paraffin embed a cynamologus monkey tibia.  It's
decalcified with the endpoint confirmed (with ammonium oxalate).  I've
had good success with mouse bones with the following schedule, but am
not sure it's long enough for this larger sample:

-        70% EtOH 1.5 hours

-        80% EtOH 1.5 hours

-        95% EtOH 2x 1.5 hours

-        100% EtOH 2x 1.5 hours

-        Clearite 3 2x1.5 hours

-        Tissue Prep 2 paraffin 4x1.5 hours 60 degrees under vacuum

-        Embed in tissue prep 2



We're hand processing, so any/all advice is welcome!!



Many thanks in advance!



d



Danielle Crippen

Morphology and Imaging Core Manager

Buck Institute for Age Research






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