[Histonet] questions re: fixing in general and Histochoice in particular

[Jacqui Detmar]

Hi all.  Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please.  Here are the
questions:  

 

1.        When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?  

2.       At what temperature should one be fixing tissues?

 

Regarding Histochoice:

 

1.        For how long should you fix the tissue?

2.       What volume ratio of fixative:tissue should you use?

3.       How long can you store Histochoice-fixed tissues in 70%
ethanol?

 

I think that's about it.  Thanks in advance,

 

Jacqui

 

Jacqui Detmar, Post-doctoral Fellow

Samuel Lunenfeld Research Institute

Mount Sinai Hospital

25 Orde Street, room 6-1001AJ

Toronto, ON   M5T 3H7

 

Email:    detmar <@t> lunenfeld.ca

Phone: 416-586-4800 x5607

Fax:        416-586-5993