[Histonet] IF

[Merced Leiker]

sorry, that should read "for up to a few weeks."

--On Tuesday, March 24, 2009 1:00 PM -0400 Merced Leiker 
<leiker <@t> buffalo.edu> wrote:

> We don't let our unfixed sections air-dry (room temp) long before fixing
> them - a few minutes? For some people here I let them sit a few minutes
> then just store them in the -80 for up to a weeks weeks before they fix
> them and commence with their staining procedure. If the animals were
> perfusion-fixed maybe they can air-dry longer. I've heard everything from
> 5 min to overnight. At room temperature.
>
> Just my input.
>
> --On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan"
> <vjp2105 <@t> columbia.edu> wrote:
>
>> Hi everyone,
>>
>> When carrying out IF on frozen sections how long after the cutting the
>> sections should you leave the slides to dry and where before starting the
>> staining procedure?
>>
>> Thanks a mill,
>>
>> V
>>
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>
>
>
> Merced M Leiker
> Research Technician II
> 354 Biomedical Research Building
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.