[Histonet] IF

[Merced Leiker]

We don't let our unfixed sections air-dry (room temp) long before fixing 
them - a few minutes? For some people here I let them sit a few minutes 
then just store them in the -80 for up to a weeks weeks before they fix 
them and commence with their staining procedure. If the animals were 
perfusion-fixed maybe they can air-dry longer. I've heard everything from 5 
min to overnight. At room temperature.

Just my input.

--On Tuesday, March 24, 2009 12:17 PM -0400 "Vanessa J. Phelan" 
<vjp2105 <@t> columbia.edu> wrote:

> Hi everyone,
>
> When carrying out IF on frozen sections how long after the cutting the
> sections should you leave the slides to dry and where before starting the
> staining procedure?
>
> Thanks a mill,
>
> V
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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