[Histonet] Murine Desmoplasia Markres

[Igor Deyneko]

Dear Histonetters!
I was wondering if anyone is working with Desmoplasia markers? I am working
with xenograft models (human tumors implanted in mice). So the result is
human tumor cells and Murine stroma. I am trying to test various desmoplasia
markers found in literature, but almost all of them are for human stroma. So
far I got Collagen and Fibronectin working beautifully, but I have problems
with the following proteins:
alpha Smooth Muscle Actin
*Desmin*
*GFAP (glial fibrillary actin protein)*
*CD 31 (so I'm switching to CD34)*
*SPARC*
I have a lot of cross-reactivity issues. If anyone knows of antibodies ,
which are capable of identifying the Murine structures, while keeping the
human tissues clean, please let me know. Any information would be highly
appreciated!
Thank you.
Sincerely,
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA 02139