[Histonet] Paraffin Block triming

Jennifer Anderson janderson <@t> halozyme.com
Thu Mar 19 13:01:05 CDT 2009


I think it depends on whether you place your cassette bottom side down
(slotted side close to the tissue) or bottom side up (open side close to
the tissue) when embedding.



The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 10:07 AM
To: hymclab; Doug Showers; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I also use mine warm........ 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Thursday, March 19, 2009 1:03 PM
To: 'Doug Showers'; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I disagree. I take my molds right out of the holding chamber and use
warm and have hardly any leakage and don't have to hardly scrape.
However, the other techs do have leakage and have to scrape alot off.
I think it is just in the embedders technique.

Dawn

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Doug
Showers
Sent: Thursday, March 19, 2009 11:37 AM
To: Histonet
Subject: RE: [Histonet] Paraffin Block triming

I think the amount of excess paraffin depends on the temperature of the
mold when you are embedding. I like my molds "cold" at room temperature
and don't have much leakage whereas techs who keep the molds "hot" have
a higher amount of leakage around the edges.

Doug Showers, MS, HT
Histology Manager
ProPath
8267 Elmbrook Dr. Suite 100
Dallas, TX 75247


To learn more about ProPath, please visit http://www.ProPathLab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shakun
Aswani
Sent: Thursday, March 19, 2009 11:03 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

Jeanine,
 We had the best Chief instructor Billie Swisher. I am so very thankful
that I got the training from that school. I do miss you guys Shakun

-----Original Message-----
From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7 <@t> cdc.gov]
Sent: Thursday, March 19, 2009 8:45 AM
To: Shakun Aswani; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

Of course Shakun....you trained me!


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: Shakun Aswani [mailto:Shakun.Aswani <@t> acologix.com]
Sent: Thursday, March 19, 2009 11:09 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I am also one of that

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I was wondering if I was the only one out there that rarely has to
scrape a block.


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from
the blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick
up the ribbon and tease the sections. No sharp edge. No electricity.

PKP






________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
that it's not only very quick, but quite accurate...



Scott wrote:
> Hi,
>    Does anybody use a paraffin block dewaxer ?  If so does it save any
time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: This e-mail communication and any attachments
may contain confidential and privileged information for the use of the
designated recipient(s) named above. If you are not the intended
recipient, you are hereby notified that you have received this
communication in error and that any review, disclosure, dissemination,
distribution or copying of it or its contents is prohibited. If you have
received this communication in error, please notify the sender at the
electronic mail address noted above and destroy all copies of this
communication and any attachments. Thank you for your cooperation.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




More information about the Histonet mailing list