[Histonet] Paraffin Block triming

Thu Mar 19 12:24:09 CDT 2009

I think we all develop our own methods based on what we were taught and what
becomes easiest or best for us.  I do agree with Gayle as the fingers get
older the use of these new devices is better and easier on us.  

We often build blocks here as a research lab with large pieces of tissue or
decal bone and very large molds or sometimes embedding "L"s.  Trimming can
be a big job and get dangerous.  I have come to love my Para Trimmer and the
ease of use for trimming and sometimes forming these blocks prior to
sectioning.  I do love cleaning multiple blocks at a time to save time when
I have average cassettes.  

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 1:07 PM
To: hymclab; Doug Showers; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I also use mine warm........ 

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Thursday, March 19, 2009 1:03 PM
To: 'Doug Showers'; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I disagree. I take my molds right out of the holding chamber and use
warm and have hardly any leakage and don't have to hardly scrape.
However, the other techs do have leakage and have to scrape alot off.
I think it is just in the embedders technique.

Dawn

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Doug
Showers
Sent: Thursday, March 19, 2009 11:37 AM
To: Histonet
Subject: RE: [Histonet] Paraffin Block triming

I think the amount of excess paraffin depends on the temperature of the
mold when you are embedding. I like my molds "cold" at room temperature
and don't have much leakage whereas techs who keep the molds "hot" have
a higher amount of leakage around the edges.

Doug Showers, MS, HT
Histology Manager
ProPath
8267 Elmbrook Dr. Suite 100
Dallas, TX 75247

To learn more about ProPath, please visit http://www.ProPathLab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shakun
Aswani
Sent: Thursday, March 19, 2009 11:03 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

Jeanine,
 We had the best Chief instructor Billie Swisher. I am so very thankful
that I got the training from that school. I do miss you guys Shakun

-----Original Message-----
From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7 <@t> cdc.gov]
Sent: Thursday, March 19, 2009 8:45 AM
To: Shakun Aswani; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

Of course Shakun....you trained me!

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: Shakun Aswani [mailto:Shakun.Aswani <@t> acologix.com]
Sent: Thursday, March 19, 2009 11:09 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I am also one of that

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I was wondering if I was the only one out there that rarely has to
scrape a block.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from
the blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick
up the ribbon and tease the sections. No sharp edge. No electricity.

PKP

________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
that it's not only very quick, but quite accurate...

Scott wrote:
> Hi,
>    Does anybody use a paraffin block dewaxer ?  If so does it save any
time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>

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