[Histonet] Paraffin Block trimming

[Merced Leiker]

I guess one has to go to histology school to learn how to do it, then (sent 
under separate cover from another histonetter...)  I 'm too researchy.  ;-)

--On Thursday, March 19, 2009 11:45 AM -0400 "Bartlett, Jeanine 
(CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:

> We have used a variety of cassettes and paraffin over the years...same
> result.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
> Sent: Thursday, March 19, 2009 11:16 AM
> To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce;
> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>
> hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base
> Molds...maybe it's the cassette design or type of wax used?  (VWR
> 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics
> Biopsy Cassettes)
>
>
> --On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine
> (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:
>
>> Don't know what to say.........I fill mine up and as long as I don't get
>> to rough with it (bump it or something where it sloshes) I don't have any
>> excess paraffin around the edges.  I use metal base molds.
>>
>>
>> Jeanine Bartlett
>> Infectious Diseases Pathology Branch
>> (404) 639-3590
>> jeanine.bartlett <@t> cdc.hhs.gov
>>
>>
>> -----Original Message-----
>> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
>> Sent: Thursday, March 19, 2009 10:56 AM
>> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce;
>> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>>
>> Now I'm interested in how you embed so as to not have to scrape...if i
>> don't add enough wax (enough to end up rising around the edges of the
>> cassette, hence the scraping later), i don't get a secure hold of the
>> block to the cassette...
>>
>> ML
>>
>> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary"
>> <gmartin <@t> marshallmedical.org> wrote:
>>
>>> No you're not the only one ... I was wondering the same thing ... why
>>> all the scraping.  It seems to me that clean embedding does the trick
>>> with a few exceptions.  G
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>>> Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58
>>> AM To: Paula Pierce; Histonet
>>> Subject: RE: [Histonet] Paraffin Block triming
>>>
>>> I was wondering if I was the only one out there that rarely has to
>>> scrape a block.
>>>
>>>
>>> Jeanine Bartlett
>>> Infectious Diseases Pathology Branch
>>> (404) 639-3590
>>> jeanine.bartlett <@t> cdc.hhs.gov
>>>
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
>>> Pierce Sent: Thursday, March 19, 2009 9:57 AM
>>> To: Histonet
>>> Subject: [Histonet] Paraffin Block triming
>>>
>>> I try to embed so as to have a minimal amount of paraffin to scrape from
>>> the blocks. ;)
>>>
>>> But, I do scrape using the handle end of the same forceps I use to pick
>>> up the ribbon and tease the sections. No sharp edge. No electricity.
>>>
>>> PKP
>>>
>>>
>>>  
>>>
>>>
>>>
>>> ________________________________
>>> From: Peter Carroll <carrolpb <@t> umdnj.edu>
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Sent: Thursday, March 19, 2009 8:30:34 AM
>>> Subject: Re: [Histonet] Paraffin Block triming
>>>
>>>> Does anybody use a paraffin block dewaxer ?
>>>
>>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
>>> that it's not only very quick, but quite accurate...
>>>
>>>
>>>
>>> Scott wrote:
>>>> Hi,
>>>>     Does anybody use a paraffin block dewaxer ?  If so does it save any
>>>> time, how well does it work?
>>>>
>>>> Thanks,
>>>>
>>>> Scott Hendricksen  HT (ASCP)
>>>> _______________________________________________
>>>> Histonet mailing list
>>>> Histonet <@t> lists.utsouthwestern.edu
>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>>
>>>>
>>>>  
>>>
>>>
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>>
>>
>>
>> Merced M Leiker
>> Research Technician II
>> 354 Biomedical Research Building
>> School of Medicine and Biomedical Sciences
>> State University of New York at Buffalo
>> 3435 Main St, Buffalo, NY 14214
>> Ph: (716) 829-6033
>> Fx: (716) 829-2725
>>
>> No trees were harmed in the sending of this email.
>> However, many electrons were severely inconvenienced.
>>
>>
>>
>>
>
>
>
> Merced M Leiker
> Research Technician II
> 354 Biomedical Research Building
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>



Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.