[Histonet] Paraffin Block trimming

[Bartlett, Jeanine (CDC/CCID/NCZVED)]

We have used a variety of cassettes and paraffin over the years...same result. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: Merced Leiker [mailto:leiker <@t> buffalo.edu] 
Sent: Thursday, March 19, 2009 11:16 AM
To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block trimming

hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used?  (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes)


--On Thursday, March 19, 2009 10:57 AM -0400 "Bartlett, Jeanine 
(CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:

> Don't know what to say.........I fill mine up and as long as I don't get
> to rough with it (bump it or something where it sloshes) I don't have any
> excess paraffin around the edges.  I use metal base molds.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: Merced Leiker [mailto:leiker <@t> buffalo.edu]
> Sent: Thursday, March 19, 2009 10:56 AM
> To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce;
> Histonet Subject: RE: [Histonet] Paraffin Block trimming
>
> Now I'm interested in how you embed so as to not have to scrape...if i
> don't add enough wax (enough to end up rising around the edges of the
> cassette, hence the scraping later), i don't get a secure hold of the
> block to the cassette...
>
> ML
>
> --On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary"
> <gmartin <@t> marshallmedical.org> wrote:
>
>> No you're not the only one ... I was wondering the same thing ... why all
>> the scraping.  It seems to me that clean embedding does the trick with a
>> few exceptions.  G
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
>> Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM
>> To: Paula Pierce; Histonet
>> Subject: RE: [Histonet] Paraffin Block triming
>>
>> I was wondering if I was the only one out there that rarely has to scrape
>> a block.
>>
>>
>> Jeanine Bartlett
>> Infectious Diseases Pathology Branch
>> (404) 639-3590
>> jeanine.bartlett <@t> cdc.hhs.gov
>>
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
>> Pierce Sent: Thursday, March 19, 2009 9:57 AM
>> To: Histonet
>> Subject: [Histonet] Paraffin Block triming
>>
>> I try to embed so as to have a minimal amount of paraffin to scrape from
>> the blocks. ;)
>>
>> But, I do scrape using the handle end of the same forceps I use to pick
>> up the ribbon and tease the sections. No sharp edge. No electricity.
>>
>> PKP
>>
>>
>>  
>>
>>
>>
>> ________________________________
>> From: Peter Carroll <carrolpb <@t> umdnj.edu>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Sent: Thursday, March 19, 2009 8:30:34 AM
>> Subject: Re: [Histonet] Paraffin Block triming
>>
>>> Does anybody use a paraffin block dewaxer ?
>>
>> Yep, it's called "my own two hands and a metal spatula", ha ha :) I find
>> that it's not only very quick, but quite accurate...
>>
>>
>>
>> Scott wrote:
>>> Hi,
>>>     Does anybody use a paraffin block dewaxer ?  If so does it save any
>>> time, how well does it work?
>>>
>>> Thanks,
>>>
>>> Scott Hendricksen  HT (ASCP)
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>>  
>>
>>
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>
>
>
> Merced M Leiker
> Research Technician II
> 354 Biomedical Research Building
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>



Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.