[Histonet] Paraffin block - cassette cleaner

Thu Mar 19 10:16:20 CDT 2009

We use the ThermoFisher model also. It keeps the floor much cleaner, too,
since the paraffin is melted into a small cup rather than flying around the
counter and floor. 

Doug Showers, MS, HT
Histology Manager
ProPath
8267 Elmbrook Dr. Suite 100
Dallas, TX 75247

To learn more about ProPath, please visit http://www.ProPathLab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kay, Karen
Sent: Thursday, March 19, 2009 10:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin block - cassette cleaner

Hello,
We have the Thermo Fisher model as well and love it.  It does an excellent
job on the blocks.in much less time than traditional hand scraping.
Karen J Kay, MLT
Pathology Supervisor,Chinook Health Laboratory
Chinook Regional Hospital,Lethbridge, Alberta, CANADA

-----Original Message-----
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Sent: March 19, 2009 9:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 64, Issue 33

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Today's Topics:

   1. equipment purchasing (Patty Dunlop)
   2. glycerol buffered antifade media (Neil M. Fournier)
   3. Double-stain for immune cell markers (Margaryan, Naira)
   4. Paraffin Block triming (Scott)
   5. Re: Paraffin Block trimming (Jennifer MacDonald)
   6. Re: Paraffin Block triming (Pamela Marcum)
   7. RE: RE: Automating muscle and neuro stains (Denise Piontek)
   8. Re: Paraffin Block trimming (Angela Bitting)
   9. Re: Paraffin Block trimming (Rene J Buesa)
  10. RE: Paraffin Block trimming (Pamela Marcum)
  11. Re: Paraffin Block triming (Peter Carroll)
  12. RE: Paraffin Block trimming (Karin Groeger)
  13. RE: Paraffin Block trimming (McMahon, Loralee A)
  14. Paraffin Block triming (Paula Pierce)
  15. RE: Paraffin Block triming (Bartlett, Jeanine (CDC/CCID/NCZVED))
  16. Re: Paraffin Block trimming (Kelly Boyd)
  17. Re: Paraffin Block trimming (godsgalnow <@t> aol.com)
  18. RE: Paraffin Block trimming (Podawiltz, Thomas)
  19. RE: Paraffin Block trimming (Martin, Gary)
  20. FW: [Histonet] RE: Automating muscle and neuro stains
      (Ian Montgomery)
  21. Sanderson's RBS Image (Suzanne Bruce)
  22. RE: Paraffin Block trimming (Merced Leiker)

----------------------------------------------------------------------

Message: 1
Date: Wed, 18 Mar 2009 10:09:40 -0700
From: Patty Dunlop <pdunlop720 <@t> gmail.com>
Subject: [Histonet] equipment purchasing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<80ab7bc60903181009o6e178432m42613f7e5cc0eb18 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello,

Our histo lab is currently looking into buying one or all of the following:

cassette labeler, automated stainer (that can also accommodate a few special
stains), and automated coverslipper (that uses minimal xylene or no xylene).

Can anyone recommend good brands and models that they like as well as what
vendors might have good prices?  We are also looking into getting the stainer
and coverslipper either used or refurbished to cut down on costs. Our lab is
small and we do only about 40-50 slides per day on average.  We would prefer
to have the equipment to be as small as possible.

Any suggestions are welcome!

Thanks,
Patty

------------------------------

Message: 2
Date: Wed, 18 Mar 2009 13:18:23 -0600
From: "Neil M. Fournier" <nfournier <@t> sasktel.net>
Subject: [Histonet] glycerol buffered antifade media
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <E742D69A2175489792EBE1CFF973C3B0 <@t> Neil45EAF11E9E>
Content-Type: text/plain; charset=us-ascii

Hi Everyone,

I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M
phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol  (pH to ~ 10.0
with NaOH)

However, I notice that solution became quite a dark yellow when dissolving.
Is this normal? It seems to occur every time I try to make it up. 

Thanks in advance

Neil

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------------------------------

Message: 3
Date: Wed, 18 Mar 2009 14:20:34 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] Double-stain for immune cell markers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<6A2230BAC92E3B4084DAE06869B89FB601E6CC03 <@t> CMHEXC01EVS.childrensmemorial.org>

Content-Type: text/plain;	charset="us-ascii"

Hi Histofriends,

I was requested to make the double-staining for immune cell markers together
with another genes (IgG goat) that our lab researching on human tumors
xenografted in mouse.

Which companies kits you will suggest to use for this experiment (the
double-staining) and, what Abs (not mouse and not goat) are the best to
represent the immune cell markers.

Respectfully,

Naira

------------------------------

Message: 4
Date: Wed, 18 Mar 2009 22:19:45 -0600
From: "Scott" <lscott <@t> sfcn.org>
Subject: [Histonet] Paraffin Block triming
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <550661ED746D44B48C04DB86EB971C75 <@t> LesliePC>
Content-Type: text/plain;	charset="iso-8859-1"

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any time,
how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP)

------------------------------

Message: 5
Date: Wed, 18 Mar 2009 21:26:37 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OFEDD221F9.07AA17F5-ON8825757E.00184C4A-8825757E.0018B972 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Thu, 19 Mar 2009 06:34:38 -0400 (EDT)
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: Re: [Histonet] Paraffin Block triming
To: Scott <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:

<1128894179.63154441237458878301.JavaMail.root <@t> zm-mbx-modv.zimbra.upenn.edu>

Content-Type: text/plain; charset=utf-8

Hi Scott,

We have one from Thermo Fisher (Shandon)and love it.  It saves time and
fingers in cleaning cassettes.  I also use it to trim and shape large blocks
for sectioning.  No shavings no razor blades. 

Pam Marcum

----- Original Message -----
From: "Scott" <lscott <@t> sfcn.org>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern
Subject: [Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any time,
how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 7
Date: Thu, 19 Mar 2009 08:28:44 -0400
From: Denise Piontek <dbpiontek <@t> hotmail.com>
Subject: RE: [Histonet] RE: Automating muscle and neuro stains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL112-W22E7AB3AD4E6CBB1857204AB960 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

 I was hoping to determine the level of interest in having muscle and neuro
stains automated? I am speaking with a vendor about the desire to automate
many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast
Blue, etc..... 

Would your lab use this technology?

Would you do this work in house instead of sending it out if it were
automated?

How many tests does your lab do per year in this category?

Would you prefer this was a feature on your automated special stainer?

I will compile the answers.

Thanks in advance for your participation.

Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC

Technical Director

Massachusetts General Hospital

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Message: 8
Date: Thu, 19 Mar 2009 09:13:46 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: <histonet-bounces <@t> lists.utsouthwestern.edu>,	"Jennifer MacDonald"
	<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <49C20CCA.2B7F.00C9.0 <@t> geisinger.edu>
Content-Type: text/plain; charset="us-ascii"

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM >>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
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TEL;WORK:570-271-6844
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------------------------------

Message: 9
Date: Thu, 19 Mar 2009 06:25:19 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu,	Jennifer MacDonald
	<JMacDonald <@t> mtsac.edu>, Scott <lscott <@t> sfcn.org>,	Angela
Bitting
	<akbitting <@t> geisinger.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <767749.47598.qm <@t> web65708.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

You will have to time worself as I did to find out that waiting fo the
paraffin to melt in order to eliminate it takes 2.5 times more time than
doing it manually with a pocket knife (as I used). Manually = 32 secs/block
vs. 80 secks./block René J.

--- On Thu, 3/19/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

From: Angela Bitting <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:13 AM

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM
>>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
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is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
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notify me immediately by replying to this email. Thank
you._______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 10
Date: Thu, 19 Mar 2009 09:31:21 -0400
From: "Pamela Marcum" <pmarcum <@t> vet.upenn.edu>
Subject: RE: [Histonet] Paraffin Block trimming
To: "'Angela Bitting'" <akbitting <@t> geisinger.edu>,
	<histonet-bounces <@t> lists.utsouthwestern.edu>,	"'Jennifer
MacDonald'"
	<JMacDonald <@t> mtsac.edu>, "'Scott'" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <000c01c9a896$fe832c60$095a5b82 <@t> vet.upenn.edu>
Content-Type: text/plain;	charset="US-ASCII"

Actually the warm up time is about 3 minutes and after that I can do blocks
much faster and cleaner than scraping.  I can't imagine it being slower to be
honest.  We love it as it save a lot of time. 

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, March 19, 2009 9:14 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Jennifer MacDonald; Scott
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM >>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it
is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank you.

------------------------------

Message: 11
Date: Thu, 19 Mar 2009 09:30:34 -0400
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] Paraffin Block triming
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49C248FA.5060906 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

 > Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find 
that it's not only very quick, but quite accurate...

Scott wrote:
> Hi,
>     Does anybody use a paraffin block dewaxer ?  If so does it save 
> any time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP) 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   

------------------------------

Message: 12
Date: Thu, 19 Mar 2009 06:37:46 -0700
From: "Karin Groeger" <KGroeger <@t> USLABS.net>
Subject: RE: [Histonet] Paraffin Block trimming
To: "Pamela Marcum" <pmarcum <@t> vet.upenn.edu>,	"Angela Bitting"
	<akbitting <@t> geisinger.edu>,
	<histonet-bounces <@t> lists.utsouthwestern.edu>,	"Jennifer MacDonald"
	<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<F5085C791AAF6F47B0170D0B84FABCCC0383A141 <@t> PRODEXCH01.uslabs.int>
Content-Type: text/plain;	charset="us-ascii"

They work great for us and saves the hand.  We use the Shandon Para Trimmer.
Our techs love them.

Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, March 19, 2009 6:31 AM
To: 'Angela Bitting'; histonet-bounces <@t> lists.utsouthwestern.edu;
'Jennifer MacDonald'; 'Scott'
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin Block trimming

Actually the warm up time is about 3 minutes and after that I can do blocks
much faster and cleaner than scraping.  I can't imagine it being slower to be
honest.  We love it as it save a lot of time. 

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, March 19, 2009 9:14 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Jennifer MacDonald; Scott
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM >>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
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is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank you.

_______________________________________________
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Message: 13
Date: Thu, 19 Mar 2009 09:45:46 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] Paraffin Block trimming
To: <rjbuesa <@t> yahoo.com>, <histonet-bounces <@t> lists.utsouthwestern.edu>,
	"Jennifer MacDonald" <JMacDonald <@t> mtsac.edu>, "Scott"
	<lscott <@t> sfcn.org>, 	"Angela Bitting" <akbitting <@t> geisinger.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:

<2CF6F6B05263EA4EBAB07781B51E5DB002C946D9 <@t> e2k3ms1.urmc-sh.rochester.edu>

Content-Type: text/plain;	charset="iso-8859-1"

In my experience the paraffin block trimmer is quicker and easier on your
hands than the old manual scraping method.

Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology

(585) 275-7210

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Thu 3/19/2009 9:25 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Jennifer MacDonald; Scott;
Angela Bitting
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

You will have to time worself as I did to find out that waiting fo the
paraffin to melt in order to eliminate it takes 2.5 times more time than
doing it manually with a pocket knife (as I used). Manually = 32 secs/block
vs. 80 secks./block René J.

--- On Thu, 3/19/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

From: Angela Bitting <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:13 AM

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM
>>>
We have two in our student lab.  It is safer, quicker, and there are a lot
less paraffin shavings to clean up. Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any time,
how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it
is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank
you._______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 14
Date: Thu, 19 Mar 2009 06:56:46 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] Paraffin Block triming
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <769731.2958.qm <@t> web1107.biz.mail.sk1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I try to embed so as to have a minimal amount of paraffin to scrape from the
blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick up
the ribbon and tease the sections. No sharp edge. No electricity.

PKP

________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find 
that it's not only very quick, but quite accurate...

Scott wrote:
> Hi,
>    Does anybody use a paraffin block dewaxer ?  If so does it save any 
>time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP) 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>  

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 15
Date: Thu, 19 Mar 2009 09:58:25 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Paraffin Block triming
To: "Paula Pierce" <contact <@t> excaliburpathology.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9A16CB5D55FC1648ADF11B63E72A1BE1BF03D4 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=iso-8859-1

I was wondering if I was the only one out there that rarely has to scrape a
block.   

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from the
blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick up
the ribbon and tease the sections. No sharp edge. No electricity.

PKP

________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that
it's not only very quick, but quite accurate...

Scott wrote:
> Hi,
>    Does anybody use a paraffin block dewaxer ?  If so does it save any 
>time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP) 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>  

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 16
Date: Thu, 19 Mar 2009 07:03:08 -0700 (PDT)
From: Kelly Boyd <kdboydhisto <@t> yahoo.com>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu,	Jennifer MacDonald
	<JMacDonald <@t> mtsac.edu>, Scott <lscott <@t> sfcn.org>,	Angela
Bitting
	<akbitting <@t> geisinger.edu>, rjbuesa <@t> yahoo.com
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <32862.36509.qm <@t> web58608.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

My techs use our Para Trimmer from Thermo Scientific Shandon. It will save
your hands, but it takes a lot more time than the old fashion hand scraping.
I prefer to hand scrape. I can scrape at least twice as many by hand than
with the para trimmer.

Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com 

Tele (252)-830-6866
Cell  (252)-943-9527
Fax  (252)-830-0032

--- On Thu, 3/19/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>, "Angela Bitting"
<akbitting <@t> geisinger.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:25 AM

You will have to time worself as I did to find out that waiting fo the
paraffin to melt in order to eliminate it takes 2.5 times more time than
doing it manually with a pocket knife (as I used). Manually = 32 secs/block
vs. 80 secks./block René J.

--- On Thu, 3/19/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

From: Angela Bitting <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:13 AM

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM
>>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it
is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank
you._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 17
Date: Thu, 19 Mar 2009 10:08:40 -0400
From: godsgalnow <@t> aol.com
Subject: Re: [Histonet] Paraffin Block trimming
To: KGroeger <@t> USLABS.net, pmarcum <@t> vet.upenn.edu,
	akbitting <@t> geisinger.edu,
histonet-bounces <@t> lists.utsouthwestern.edu,
	JMacDonald <@t> mtsac.edu,	lscott <@t> sfcn.org
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CB76B3572A802B-1288-30F1 <@t> webmail-md03.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

I agree that this is quicker than manually---you can do 4?or 5 blocks at a
time.

-----Original Message-----
From: Karin Groeger <KGroeger <@t> USLABS.net>
To: Pamela Marcum <pmarcum <@t> vet.upenn.edu>; Angela Bitting
<akbitting <@t> geisinger.edu>; histonet-bounces <@t> lists.utsouthwestern.edu;
Jennifer MacDonald <JMacDonald <@t> mtsac.edu>; Scott <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Sent: Thu, 19 Mar 2009 9:37 am
Subject: RE: [Histonet] Paraffin Block trimming

They work great for us and saves the hand.  We use the Shandon Para Trimmer.
Our techs love them.

Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, March 19, 2009 6:31 AM
To: 'Angela Bitting'; histonet-bounces <@t> lists.utsouthwestern.edu;
'Jennifer MacDonald'; 'Scott'
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin Block trimming

Actually the warm up time is about 3 minutes and after that I can do blocks
much faster and cleaner than scraping.  I can't imagine it being slower to be
honest.  We love it as it save a lot of time. 

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, March 19, 2009 9:14 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Jennifer MacDonald; Scott
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM >>>
We have two in our student lab.  It is safer, quicker, and there are a lot 
less paraffin shavings to clean up.
Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anyb
ody use a paraffin block dewaxer ?  If so does it save any 
time, how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it
is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank you.

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Confidentiality Notice: This message, including any attachments, may contain 
CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is
intended 
for use by the individual named above and may not be disseminated to any
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party without US LABS' written permission. If you are not the intended 
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------------------------------

Message: 18
Date: Thu, 19 Mar 2009 10:24:00 -0400
From: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>
Subject: RE: [Histonet] Paraffin Block trimming
To: Kelly Boyd <kdboydhisto <@t> yahoo.com>,
	"histonet-bounces <@t> lists.utsouthwestern.edu"
	<histonet-bounces <@t> lists.utsouthwestern.edu>, Jennifer MacDonald
	<JMacDonald <@t> mtsac.edu>, Scott <lscott <@t> sfcn.org>, Angela Bitting
	<akbitting <@t> geisinger.edu>, "rjbuesa <@t> yahoo.com" <rjbuesa <@t> yahoo.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<38667E7FB77ECD4E91BFAEB8D98638631D33B5BD20 <@t> LRGHEXVS1.practice.lrgh.org>

Content-Type: text/plain; charset="iso-8859-1"

We have one timmer. I don't use it, I hand trim simply because I am quicker
with it. The other techs use the timmer to save their hands.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
[kdboydhisto <@t> yahoo.com]
Sent: Thursday, March 19, 2009 10:03 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu; Jennifer MacDonald; Scott;
Angela Bitting; rjbuesa <@t> yahoo.com
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block trimming

My techs use our Para Trimmer from Thermo Scientific Shandon. It will save
your hands, but it takes a lot more time than the old fashion hand scraping.
I prefer to hand scrape. I can scrape at least twice as many by hand than
with the para trimmer.

Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com

Tele (252)-830-6866
Cell  (252)-943-9527
Fax  (252)-830-0032

--- On Thu, 3/19/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>, "Angela Bitting"
<akbitting <@t> geisinger.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:25 AM

You will have to time worself as I did to find out that waiting fo the
paraffin to melt in order to eliminate it takes 2.5 times more time than
doing it manually with a pocket knife (as I used). Manually = 32 secs/block
vs. 80 secks./block René J.

--- On Thu, 3/19/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

From: Angela Bitting <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Paraffin Block trimming
To: histonet-bounces <@t> lists.utsouthwestern.edu, "Jennifer MacDonald"
<JMacDonald <@t> mtsac.edu>, "Scott" <lscott <@t> sfcn.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 19, 2009, 9:13 AM

I've been told that they are slower than scraping by hand. What's the
consensus?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> 3/19/2009 12:26 AM
>>>
We have two in our student lab.  It is safer, quicker, and there are a lot
less paraffin shavings to clean up. Jennifer MacDonald

"Scott" <lscott <@t> sfcn.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/18/2009 09:24 PM

To
<Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Paraffin Block triming

Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any time,
how well does it work?

Thanks,

Scott Hendricksen  HT (ASCP) _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure, copying,
distribution or any action taken, or omitted to be taken, in reliance on it
is prohibited and may be unlawful. If you have received this message in
error, please delete all electronic copies of this message (and the documents
attached to it, if any), destroy any hard copies you may have created and
notify me immediately by replying to this email. Thank
you._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential
information intended only for the use of the recipient(s) named above. If you
are not the intended recipient, you may not print,distribute, or copy this
message or any attachments.  If you have received this communication in
error, please notify the sender by return e-mail and delete this message and
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LRGHealthcare.

------------------------------

Message: 19
Date: Thu, 19 Mar 2009 07:37:13 -0700
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: RE: [Histonet] Paraffin Block trimming
To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <jqb7 <@t> cdc.gov>,	"Paula
	Pierce" <contact <@t> excaliburpathology.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<6ED9D4252F278841A0593D3D788AF24C04D283C8 <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain;	charset="iso-8859-1"

No you're not the only one ... I was wondering the same thing ... why all the
scraping.  It seems to me that clean embedding does the trick with a few
exceptions. 
G

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I was wondering if I was the only one out there that rarely has to scrape a
block.   

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from the
blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick up
the ribbon and tease the sections. No sharp edge. No electricity.

PKP

________________________________
From: Peter Carroll <carrolpb <@t> umdnj.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

> Does anybody use a paraffin block dewaxer ?

Yep, it's called "my own two hands and a metal spatula", ha ha :) I find that
it's not only very quick, but quite accurate...

Scott wrote:
> Hi,
>    Does anybody use a paraffin block dewaxer ?  If so does it save any 
>time, how well does it work?
>
> Thanks,
>
> Scott Hendricksen  HT (ASCP) 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>  

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------------------------------

Message: 20
Date: Thu, 19 Mar 2009 14:38:58 -0000
From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
Subject: FW: [Histonet] RE: Automating muscle and neuro stains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <57D70B824966452FA0D788FD34277E38 <@t> IBLS.GLA.AC.UK>
Content-Type: text/plain;	charset="us-ascii"

	Have I died and gone to histologists heaven? Automate an ATPase, now
there would be something special. No more crying and wailing over failed or
partially successful acid reversal. I'll have to retreat to a darkened room,
I'm traumatised. 
Ian.

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.

Subject: RE: [Histonet] RE: Automating muscle and neuro stains

 I was hoping to determine the level of interest in having muscle and neuro
stains automated? I am speaking with a vendor about the desire to automate
many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast
Blue, etc..... 

Would your lab use this technology?

Would you do this work in house instead of sending it out if it were
automated?

How many tests does your lab do per year in this category?

Would you prefer this was a feature on your automated special stainer?

I will compile the answers.

Thanks in advance for your participation.

Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC

Technical Director

Massachusetts General Hospital

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------------------------------

Message: 21
Date: Thu, 19 Mar 2009 10:55:20 -0400
From: "Suzanne Bruce" <sbruce <@t> vetpathservicesinc.com>
Subject: [Histonet] Sanderson's RBS Image
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<26DB1FDFBF9EE14AB3AACC873519A4A2855191 <@t> vpss1.VetPathServicesInc.local>

Content-Type: text/plain;	charset="iso-8859-1"

Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid
Bone should look like?

Thanks in advance,
Suzanne

_______________________________
Suzanne Bruce,  R.V.T.
Histologist & Necropsy Coordinator
<https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesin
c.com/>  

------------------------------

Message: 22
Date: Thu, 19 Mar 2009 10:55:36 -0400
From: Merced Leiker <leiker <@t> buffalo.edu>
Subject: RE: [Histonet] Paraffin Block trimming
To: "Martin, Gary" <gmartin <@t> marshallmedical.org>,	"Bartlett, Jeanine
	(CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>,	Paula Pierce
	<contact <@t> excaliburpathology.com>, 	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1E3C94C039A525739E0774E6 <@t> bchwxp2702.ad.med.buffalo.edu>
Content-Type: text/plain; charset=iso-8859-1; format=flowed

Now I'm interested in how you embed so as to not have to scrape...if i 
don't add enough wax (enough to end up rising around the edges of the 
cassette, hence the scraping later), i don't get a secure hold of the block 
to the cassette...

ML

--On Thursday, March 19, 2009 7:37 AM -0700 "Martin, Gary" 
<gmartin <@t> marshallmedical.org> wrote:

> No you're not the only one ... I was wondering the same thing ... why 
> all the scraping.  It seems to me that clean embedding does the trick 
> with a few exceptions.  G
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 
> 6:58 AM
> To: Paula Pierce; Histonet
> Subject: RE: [Histonet] Paraffin Block triming
>
> I was wondering if I was the only one out there that rarely has to 
> scrape a block.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula 
> Pierce Sent: Thursday, March 19, 2009 9:57 AM
> To: Histonet
> Subject: [Histonet] Paraffin Block triming
>
> I try to embed so as to have a minimal amount of paraffin to scrape 
> from the blocks. ;)
>
> But, I do scrape using the handle end of the same forceps I use to 
> pick up the ribbon and tease the sections. No sharp edge. No 
> electricity.
>
> PKP
>
>
>  
>
>
>
> ________________________________
> From: Peter Carroll <carrolpb <@t> umdnj.edu>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Thursday, March 19, 2009 8:30:34 AM
> Subject: Re: [Histonet] Paraffin Block triming
>
>> Does anybody use a paraffin block dewaxer ?
>
> Yep, it's called "my own two hands and a metal spatula", ha ha :) I 
> find that it's not only very quick, but quite accurate...
>
>
>
> Scott wrote:
>> Hi,
>>     Does anybody use a paraffin block dewaxer ?  If so does it save 
>> any time, how well does it work?
>>
>> Thanks,
>>
>> Scott Hendricksen  HT (ASCP) 
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu 
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>  
>
>
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>
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>
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu 
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>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu 
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>

Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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