[Histonet] glycerol buffered antifade media

Neil M. Fournier nfournier <@t> sasktel.net
Wed Mar 18 14:18:23 CDT 2009

Hi Everyone,


I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M
phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol  (pH to ~ 10.0
with NaOH)

However, I notice that solution became quite a dark yellow when dissolving.
Is this normal? It seems to occur every time I try to make it up. 


Thanks in advance





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