Re: Re: [Histonet] BrdU immunostain on rat brain section

[TF]

Abcam monoclonal rat-anti BrdU

http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html 

Just compare to other Abcam anti-BrdU, it has most citations at least.

2009-03-18 



TF 



发件人： Amy Lee 
发送时间： 2009-03-18  07:14:56 
收件人： tifei 
抄送： 
主题： Re: [Histonet] BrdU immunostain on rat brain section 
 
Hi,
Could you please tell me your antibody catalog #? 

Thanks again,
Amy

--- On Mon, 3/16/09, TF <tifei <@t> foxmail.com> wrote:

From: TF <tifei <@t> foxmail.com>
Subject: Re: [Histonet] BrdU immunostain on rat brain section
To: "amylee779" <amylee779 <@t> yahoo.com>, "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, March 16, 2009, 7:10 PM


 
hi, we worked a lot on this.
I prefer to use 40 um frozen sections.
Abcam anti-BrdU (rat sourced) is the best ever I know. It does not have high background with rat IgG. Some other mouse-/sheep- sourced BrdU are available.


2009-03-17 



TF 



发件人： Amy Lee 
发送时间： 2009-03-17  05:02:28 
收件人： histonet 
抄送： 
主题： [Histonet] BrdU immunostain on rat brain section 
Hello,
I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 
1.Do you prefer frozen or paraffin? 
2.Which antibody you recommend?
3.營f牋35um frozen is requied, what thickness you will use if you want go for paraffin?
?
?
Thanks,
Amy
      
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