[Histonet] 20 micron resin sections

[Patsy Ruegg]

I have cut 20 micron sections of GMA resin using tungsten carbide knives,
the cutting is not so difficult (depending on what you are cutting ie
calcified bone) as adhering the thick sections to a slide.  Thinner sections
will stick to + slides a lot better than the thick sections will.  So unless
they are planning on float staining this will be an obstacle.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
renton
Sent: Saturday, March 14, 2009 12:48 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] 20 micron resin sections

Thanks all,

This is more or less what i thought, that 20mu sections  would prove to be
difficult and not all that feasible. So, I wait with bated breath for the
protocol from the researcher............


On 3/13/09, Peggy Bisher <mbisher <@t> princeton.edu> wrote:
>
> One of the labs here use JB4 to section Zebrafish. I sent your question to
> them to see if they could help you out. Here is their response:
>
> The consensus in the lab (Kari and I) is that no, probably not.  The size
> would probably shred the section and chip it to where they would be
uneven,
> etc.  Probably cryo or vibratome would be best.
>
> They routinely cut their sections between 2-5 microns.
>
> Good luck to you!
>
> Cheers,
>
> Margaret E. Bisher
> Electron Microscopy & Histology Core Facility Manager
> Department of Molecular Biology
> Princeton University
> Moffett Laboratory, Room 113
> Princeton, New Jersey
> Office: (609) 258-7026
> Fax: (609) 258-8468
> mbisher <@t> princeton.edu
>
>
>
>
>
> On 3/13/09 3:53 AM, "louise renton" <louise.renton <@t> gmail.com> wrote:
>
> > Hi all,
> >
> > I have a query from a colleague doing research on neuroanatomy as to
> whether
> > it is possible ( with relative ease)  to cut 20mu sections from JB4
resin
> > embedded tissue? Apparently these sections ae to be stained and then
used
> > for stereomicroscopy. My experience is not that extensive to be able to
> > answer her, so I would appreciate some advice here
> > best regards--
> > Louise Renton
> > Bone Research Unit
> > University of the Witwatersrand
> > Johannesburg
> > South Africa
> > "There are nights when the wolves are silent and only the moon howls".
> > George Carlin
> > No trees were killed in the sending of this message.
> > However, many electrons were terribly inconvenienced.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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