[Histonet] 20 micron resin sections

[louise renton]

Thanks all,

This is more or less what i thought, that 20mu sections  would prove to be
difficult and not all that feasible. So, I wait with bated breath for the
protocol from the researcher............


On 3/13/09, Peggy Bisher <mbisher <@t> princeton.edu> wrote:
>
> One of the labs here use JB4 to section Zebrafish. I sent your question to
> them to see if they could help you out. Here is their response:
>
> The consensus in the lab (Kari and I) is that no, probably not.  The size
> would probably shred the section and chip it to where they would be uneven,
> etc.  Probably cryo or vibratome would be best.
>
> They routinely cut their sections between 2-5 microns.
>
> Good luck to you!
>
> Cheers,
>
> Margaret E. Bisher
> Electron Microscopy & Histology Core Facility Manager
> Department of Molecular Biology
> Princeton University
> Moffett Laboratory, Room 113
> Princeton, New Jersey
> Office: (609) 258-7026
> Fax: (609) 258-8468
> mbisher <@t> princeton.edu
>
>
>
>
>
> On 3/13/09 3:53 AM, "louise renton" <louise.renton <@t> gmail.com> wrote:
>
> > Hi all,
> >
> > I have a query from a colleague doing research on neuroanatomy as to
> whether
> > it is possible ( with relative ease)  to cut 20mu sections from JB4 resin
> > embedded tissue? Apparently these sections ae to be stained and then used
> > for stereomicroscopy. My experience is not that extensive to be able to
> > answer her, so I would appreciate some advice here
> > best regards--
> > Louise Renton
> > Bone Research Unit
> > University of the Witwatersrand
> > Johannesburg
> > South Africa
> > "There are nights when the wolves are silent and only the moon howls".
> > George Carlin
> > No trees were killed in the sending of this message.
> > However, many electrons were terribly inconvenienced.
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
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>
>


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.