[Histonet] IHC stainer

[Greg Dobbin]

I echo everything Julie said! 
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


>>> Julie Trejo <jtrejo2 <@t> slu.edu> 3/13/2009 4:41:06 PM >>>
I suggest using the Leica Bond-Max.  I, an HT(ASCP)cm, did not have any
IHC
experiance, but the Bond-Max makes it happen.  Just putting dried
slides on,
patient info in the computer, slides labeled and then in 3-4 hours,
it's all
done.  Just dehydrate and clear.  Very simple and now giving me time
to
learn IHC and troubleshooting.  Some of the antibodies come "Ready to
use"
from Leica, and we titer others that aren't "Ready to use" (Biocare,
Dako
etc).
   I've seen co-workers in the past struggling with immuno's using the
Dako,
and taking alot of time and still counter-staining by hand.  The
Bond-Max
allows us (just three of us) to do all routine H&E's, special stains,
recuts, deepers and Immuno's in one day. Not bad.

Julie
On Fri, Mar 13, 2009 at 2:16 PM, Stephanie Weaver
<sweaver <@t> tvmdl.tamu.edu>wrote:

> I am in a veterinary diagnostic lab.  In the past we have had very
few
> requests for IHC and have always sent slides out to another lab to
perform
> IHC as needed.  It is time for us to start doing our own and join the
modern
> age.  We have several certified technicians, but none have experience
with
> IHC and we typically have a relatively high turnover rate. 
Therefore, I am
> hoping to be able to buy an automated stainer.  In the past most
people on
> the list seemed to be very happy with the Dako autostainer, but this
past
> week has brought so many bad remarks about Dako's service that I am
> reconsidering.  We probably will not need a high capacity
autostainer, but I
> would like walk-away capability with an easy to use system.  It will
need to
> accept other companies reagents, since veterinary infectious disease
> antibodies aren't often sold by the major companies.  Also, cost is
an issue
> and I would like to be able to bargain shop for reagents through
other
> companies.  Does anyone have any recommendations, or warnings as to
what to
> avoid?
>
> In a related issue, where do other animal tissue people get their
> antibodies for infectious diseases, e.g. Parvovirus, canine
distemper, or
> FIP?
>
> Thanks for the advice!
>
>
>
>
> Stephanie Weaver
> Texas Veterinary Medical Diagnostic Lab
>
>
>
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>



-- 
Julie Trejo, HT(ASCP)cm

Saint Louis University
Department of Dermatology
314-256-3413
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