[Histonet] Need Advice on Low Temp Tissue Processing

[Sean McBride]

Hi folks,

I have a rabbit cranial defect study in which the implant material is
sensitive to 100% ethanol at temperatures > 20*C.  As I cannot use my my
Sakura tissue processor for the specimens (min operating temp=35*C), I plan
to process them by hand @ 4*C in a cold room.  My intuition tells me that at
lower temperatures, the processing times in each solution should be extended
by a percentage, but by how much, I have not an idea.  Does anyone have any
experience or ideas with regards to low temperature tissue processing times?

Best regards,


~Sean McBride


Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-901-7540 (m)
412-268-8641 (fax)
smcbride <@t> cs.cmu.edu