[Histonet] histological stain for pancreatic beta cells

[Geoff McAuliffe]

Aldehyde fuchsin! Much simpler than Mallory Azan and very specific. You 
will need paraldehyde, a controlled substance, to make this stain. 
Alternatively, you can use acetaldehyde but you will need to triple the 
amount to get the molar equivalents correct. See "Staining properties of 
Aldehyde fuchsin analogues" by Buehner, Nettleton and Longley, J. 
Histochem. Cytochem.
27(3):782-787, 1979.

Geoff

Michele Wich wrote:
> Can I please get some suggestions for histological stains that
> demonstrate pancreatic beta cells? I need something that would be highly
> specific without using an IHC method. 
>
> Any advice is greatly appreciated! 
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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