[Histonet] Histology for Zebrafish

[Andrea Hooper]

Have you checked out the ton of protocols (including histo) available 
at ZFIN online:

http://zfin.org/zf_info/zfbook/zfbk.html



>Hi,
>I need to work on zebrafish for the first time and would like to get some
>general protocols for fixation and IHC staining.
>My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and
>sections didn't come out right (falling off & not preserved well).
>I am going to increase the time and also try 10% formalin over night. Please
>let me know if formalin works ok for IHC later.
>Thank you.
>Judy
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