[Histonet] Histology for Zebrafish

Yeonju Shim shimjudy <@t> gmail.com
Fri Mar 6 12:46:16 CST 2009


Hi,
I need to work on zebrafish for the first time and would like to get some
general protocols for fixation and IHC staining.
My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and
sections didn't come out right (falling off & not preserved well).
I am going to increase the time and also try 10% formalin over night. Please
let me know if formalin works ok for IHC later.
Thank you.
Judy


More information about the Histonet mailing list