[Histonet] Histology for Zebrafish

Yeonju Shim shimjudy <@t> gmail.com
Fri Mar 6 12:46:16 CST 2009

I need to work on zebrafish for the first time and would like to get some
general protocols for fixation and IHC staining.
My investigator used 4% PFA for 2 hrs at RT for a whole zebrafish and
sections didn't come out right (falling off & not preserved well).
I am going to increase the time and also try 10% formalin over night. Please
let me know if formalin works ok for IHC later.
Thank you.

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