[Histonet] RE: vector blue- xylene soluble- any alternatives

[C.M. van der Loos]

Hi Patsy and Anjan Kumar,
VectaMount is not aqueous, but an organic mountant too. It does not contain alcohol or xylene, in fact it hardly smells. It certainly does not mix with water. We use this mountant by washing the slide with distilled water and then let it dry completely at a 50C hotplate. Coverslip and leave it at the hotplate for an hour or so to let it harden a bit. Vectamount is not getting hard like DPX.
Cheers, 
Chris
 
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
 
Date: Wed, 4 Mar 2009 14:38:57 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] vector blue- xylene soluble- any alternatives
To: "'anjan kumar'" <drvet_anjan <@t> hotmail.com>,

If I am not mistaken vectamount is an aqueous mountant, dpx is
xylene/toluene based and requires going thru alcohols and xylene to mount.
One thing I do and you can try it, is airdry the slides and the use
permanent mounting media without going thru alcohols and xylene, keep in
mind there is a little solvent in the media so it may have an effect.  I do
this with Fast Red substrate chromogen for AP have not tried it on vector
blue.  The other option is to use an aqueous mountant and seal the coverslip
edges with clear nail polish.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anjan kumar
Sent: Wednesday, March 04, 2009 12:05 PM
To: histonet-requests <@t> lists.utsouthwestern.edu; triple immunohistochem
Subject: [Histonet] vector blue- xylene soluble- any alternatives


hello everyone,

                    i have a problem which i dont know how to tackle, the
problem is that i have purchased vector blue as a chromogen and it is xylene
soluble can anyone suggest me how to avoid this.... as funds are low i cant
purchase xylene substitutes. 

 
second question been, ...sorry this has been a addition ......
what is the difference between the DPX mountant and other permanent mounting
media like vectamount. kindly answer these questions...

 
regards,

Anjan Kumar
Junior research scientist
Veterinary Pathology
Madras veterinary college.