[Histonet] Section thickness

[Merced Leiker]

...I just want to add, I could see it being a problem only in the sense 
that thicker sections tend to give more background in your 
immunostaining...however, you could conversely get greater signal strength 
as well...

--On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan" 
<vjp2105 <@t> columbia.edu> wrote:

> Hi Guys,
>
> Just wondering what thickness you cut sections at? I was always used to
> cutting at 2-3 microns in my last lab, however in my new place they are
> cutting at 6 microns (for both H & Es and IHC), which seems to me as
> really quite thick! What would be the average cutting thickness?
>
> Thanks a mill,
>
> Vanessa
>
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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