[Histonet] DAB substrate buffer

[Patti Loykasek]

Hi Dr. Kumar. We use a 0.05M Tris buffer pH 7.7-7.9. We add 3% H2O2 right
before use. We use our own stock DAB. Perhaps your DAB was too dilute? I
hope this info helps.  If you would like more specifics, you can contact me.


Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA

> 
> hi every one,
>                   I am using Labvision HRP polymer secondary antibody with DAB
> chromogen, funny thing is that i have exhausted the DAB substrate buffer. so i
> tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing worked.
> can anyone tell me a protocol or recipe for preparing DAB substrate buffer.
> 
> Regards,
> Dr. Anjan Kumar.K.R
> 
> M.V.Sc Scholar
> 
> Dept. of Veterinary Pathology
> 
> Madras Veterinary College
> 
> Chennai-7
> 
> India
> 
> email: drvet_anjan <@t> hotmail.com
> 
> Phone: +91-9940475801
> 
> 
> 
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