[Histonet] DAB substrate buffer

Patti Loykasek ploykasek <@t> phenopath.com
Fri Jun 26 14:18:30 CDT 2009

Hi Dr. Kumar. We use a 0.05M Tris buffer pH 7.7-7.9. We add 3% H2O2 right
before use. We use our own stock DAB. Perhaps your DAB was too dilute? I
hope this info helps.  If you would like more specifics, you can contact me.

Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath Laboratories
Seattle, WA

> hi every one,
>                   I am using Labvision HRP polymer secondary antibody with DAB
> chromogen, funny thing is that i have exhausted the DAB substrate buffer. so i
> tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing worked.
> can anyone tell me a protocol or recipe for preparing DAB substrate buffer.
> Regards,
> Dr. Anjan Kumar.K.R
> M.V.Sc Scholar
> Dept. of Veterinary Pathology
> Madras Veterinary College
> Chennai-7
> India
> email: drvet_anjan <@t> hotmail.com
> Phone: +91-9940475801
> _________________________________________________________________
> Live Search extreme As India feels the heat of poll season, get all the info
> you need on the MSN News Aggregator
> http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx____
> ___________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.

More information about the Histonet mailing list