[Histonet] RE: re: control slide storage

Helen Fedor hfedor <@t> jhmi.edu
Thu Jun 25 16:00:43 CDT 2009


Hello, We have a project that has worked on this topic using TMA's and have found that storing unstained, and unbaked slides at -20 enhances the preservation of antigenicity of the samples. It is not yet published but is getting written up. TMA slides that were stored for 5 years at -20 in very small Ziploc bags were compared to freshly cut TMA slides of the same block. Surprisingly some of the freezer slides tissue stained better then the freshly cut slides. So we need to start to store our tissue blocks in the cold as well.

--
Helen L. Fedor 

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renko, Heather D.
Sent: Thursday, June 25, 2009 4:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re: control slide storage

Anyone have any  published literature for reference on storage control; refrigerator vs. room temp vs. freezer.  I have a friend asking for this and would also would love to get this information for my own reference  I searched the archives but, came up with only one publication.  Thank you in advance.

Heather D. Renko, Histology Supervisor
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko <@t> osfhealthcare.org


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