[Histonet] Rat anti-L1 Chemicon

[shymaa shawadfy]

Dear all
I need some advice concerning immunostaining with Anti-Neural Cell Adhesion
Molecule L1 from chemicon on P0 mice brains. I am trying to use this
antibody for formalin fixed paraffin embedded sections but I can not get any
signal until now, I tried antigen retrieval with 10 mM citrate buffer and I
tried different antibody concentrations.

I will appreciate any comment

Thanks a lot
Shymaa