[Histonet] Re: Histonet Digest, Vol 67, Issue 17
Awais Babri
a.babri <@t> uq.edu.au
Wed Jun 17 18:14:25 CDT 2009
From: <histonet-request <@t> lists.utsouthwestern.edu>
Reply-To: <histonet <@t> lists.utsouthwestern.edu>
Date: Thu, 18 Jun 2009 03:12:15 +1000
To: <histonet <@t> lists.utsouthwestern.edu>
Conversation: Histonet Digest, Vol 67, Issue 17
Subject: Histonet Digest, Vol 67, Issue 17
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Today's Topics:
1. PR and ER staining in dog uterus (Karen Doty)
2. Re: Inactivation of DAB (Sherwood, Margaret )
3. RE: Research Genetics (Reynolds,Donna M)
4. Looking for NYU contact (Angela Bitting)
5. Re: Re: Inactivation of DAB (Lynette Pavelich)
6. RE: Re: Inactivation of DAB (CHRISTIE GOWAN)
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Message: 1
Date: Tue, 16 Jun 2009 15:10:06 -0500
From: Karen Doty <k.fd <@t> live.com>
Subject: [Histonet] PR and ER staining in dog uterus
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL104-W3115715881A9AEB048FA6CE43F0 <@t> phx.gbl>
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Hi, Folks,
We are starting to work on a project that requires PR and ER staining in dog
uterus. I'm hoping someone will share some tips such as which antibodies
work well in dog (clone, manufacturer), and any specific special procedures
that might help, such as which antigen retrieval methods work best. I've
tried a couple antibodies already without good results in dog although they
worked very well in a mouse control.
All suggestions and advice is appreciated. Thanks very much.
Karen Doty
Dept. of Veterinary Biosciences
University of Illinois
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Message: 2
Date: Wed, 17 Jun 2009 09:30:55 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: [Histonet] Re: Inactivation of DAB
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9703E23AF7 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
We have started doing routine immunohistochemistry on our stainer and have a
large waste container that includes diaminobenzidine (DAB).
We would like to inactivate it so we don't have to pay to have the waste
removed. Can anyone help me? What do people use to inactivate their DAB?
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
The information in this e-mail is intended only for the person to whom it is
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------------------------------
Message: 3
Date: Wed, 17 Jun 2009 08:33:15 -0500
From: "Reynolds,Donna M" <dreynold <@t> mdanderson.org>
Subject: [Histonet] RE: Research Genetics
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<785BBF0C5F49CE41BA74460A43A08F0214492216E7 <@t> DCPWVMBXC0VS3.mdanderson.edu>
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Research Genetics has merged a couple of time the last few years. They are
now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the
Research Genetics on the Web but last I knew products were still available.
You can call them to get current catalog number and prices.
Donna Reynolds HT (ASCP)
Chief Histology Laboratory
U. T. M.D. Cancer Center
Cancer Biology Core Immunohisto Lab
713-792-8106
Message: 2
Date: Mon, 15 Jun 2009 14:52:39 -0400
From: "Joanne Mauger" <MAUGER <@t> email.chop.edu>
Subject: [Histonet] Re: research genetics/phoenix biotech
To: <jluis.palazon <@t> icman.csic.es>,<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A3660370200003100007C32 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII
Hi everyone,
I am unable to find this company anymore. Does anyone know if old research
genetics products are still being sold? I don't want Invitrogen- they did
away with the product I want-MicroHyb.
Thanks,
Jo Mauger
------------------------------
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Message: 4
Date: Wed, 17 Jun 2009 09:50:09 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] Looking for NYU contact
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A38BC51.2B7F.00C9.0 <@t> geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
Just wondering if anyone can give me contact information for someone in the
Neuropath Lab at NYU?
Thanks
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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FN:Bitting, Angela
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Message: 5
Date: Wed, 17 Jun 2009 10:05:13 -0400
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: Re: [Histonet] Re: Inactivation of DAB
To: <histonet <@t> lists.utsouthwestern.edu>,<MSHERWOOD <@t> PARTNERS.ORG>
Message-ID: <4A38BFD9020000EE0002A178 <@t> smtp-gw.hurleymc.com>
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We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.
1. Prepare the following aqeous stock solutions:
a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter)
2. Dilute DAB solution of necessary so that its concentration does not
exceed 0.9 mg/ml.
3. For each 10ml of DAB solution, add:
a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter)
b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%)
4. Allow mixture to stand for at least 10 hours. It is now
non-mutagenic.
5. Decolorize the mixture with ascorbic acid (add powder until color
disappears). This too is an oxidation/reduction reaction.
6. Neutralize the decolorized mixture with sodium bicarbonate (test with
pH meter, pH paper or dipsticks). Note: we recommend using magnesium
hydroxide/oxide instead of sodium bicarbonate.
7. Discard the solution down the drain provided that local wastewater
authorities have given their approval.
Hope this helps,
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
MSH Competency Coordinator
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
email: Lpaveli1 <@t> hurleymc.com
ph: 810-257-9948
fax: 810-762-7082
>>> "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG> 06/17/09 9:30 AM >>>
We have started doing routine immunohistochemistry on our stainer and
have a
large waste container that includes diaminobenzidine (DAB).
We would like to inactivate it so we don't have to pay to have the waste
removed. Can anyone help me? What do people use to inactivate their
DAB?
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (W224)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
The information in this e-mail is intended only for the person to whom
it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you
in error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Wed, 17 Jun 2009 14:27:39 +0000
From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
Subject: RE: [Histonet] Re: Inactivation of DAB
To: <msherwood <@t> partners.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT110-W259F909487498CDB4F48A8AE3C0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi Peggy,
You wrote:
> We have started doing routine immunohistochemistry on our stainer and have a
> large waste container that includes diaminobenzidine (DAB).
>
> We would like to inactivate it so we don't have to pay to have the waste
> removed. Can anyone help me? What do people use to inactivate their DAB?
May I suggest that you access the histonet archives where you will be able
to see all the previous discussions on this topic.
The responses were many and varied depending on different lab types and
locations. Good luck with your search.
Respectfully,
Christie Gowan
Surgical Pathology
University of Alabama Hospital
------------------------------
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End of Histonet Digest, Vol 67, Issue 17
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