[Histonet] Hif-1 alpha

[John Shelley]

I am in need of some help with Hif-1 alpha that was purchased through Novus. The tissue I am working with is mouse breast cancer and mouse lung cancer and the tissue is frozen and fixed in acetone for 10 mins. I am not getting any specific staining because my Negative Control looks just like my Positive Control tissue. I have tried no treatment, Protease 2, pH 8.4 with heat and no heat through the process on a Ventana Discovery XT.  I need some one's protocol or recommendations as to how I should proceed with additional optimizing. Thanks!!

John