[Histonet] microtome calibration

[Charles Scouten]

It is not a "calibration" issue.  Most likely cause it too shallow a blade angle, another is something is loose and wobbly.  Try grasping the blade holder and see if it can be moved in any direction.  The tissue pedestal.  If something is loose, that is the cause.

Cordially,
Charles W. Scouten, Ph.D
Innovation Manager
Biosystems Division

Leica Biosystems St. Louis LLC
5918 Evergreen Blvd.
St. Louis, MO 63134
United States of America

telephone +1 314 522 0300   Ext. 342
facsimile +1 630 964 0576
www.MyNeuroLab.com
www.leica-microsystems.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dianne Holmes
Sent: Tuesday, June 02, 2009 4:44 PM
To: Histonet
Subject: [Histonet] microtome calibration

Has anyone had problems with the calibration of an A/O Spencer sliding microtome?  I have used this one for years and now I am getting 'thick-thin' sections?  I thought maybe the accumulation of oils in the mechanics of it have slowed things down.  Can WD40 be applied without adverse effects?  I certainly would not attempt opening up all the gears!!  Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed.


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