[Histonet] Chris - T.Blue/nerve tissue

Vicki Kalscheur kalschev <@t> svm.vetmed.wisc.edu
Mon Jun 8 09:23:18 CDT 2009

Chris,  If you do not do this, consider etching your tissue to remove the surface plastic. We use 0.2% formic acid - start with less than a minute as your tissues are thin.  Some labs etch using ethyl alcohol or methanol.  We have success and good contrast with a pH less than 5. For thinner sections, I have used a T.Blue 0.1 % Aq for Metachromasia from the PolyScientific Co.   Rinse well with water and dip into 90% at the end ( give great contrast).  Vicki

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