[Histonet] immunohistochemistry

[Sally Price]

Hatem:
Since this info hasn't already been offered, I'd suggest that you
considering using a 'programmable' water bath or pressure cooker -- where
the temperature is reduced to 65 to 75 degrees, but the exposure time
extended to three to six hours.
Sally

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Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT)
From: Hatem Salim <dr.hatemsaied <@t> yahoo.com>
Subject: [Histonet] immunohistochemistry
To: histonet <@t> lists.utsouthwestern.edu

HI
I am Research assistant at Physiology department of Michigan state
university. I am using the (ß-Catenin Antibody (Carboxy-terminal Antigen)
#9587 for IHC on mice femur sections. I have used this antigen unmasking
method: For Citrate: Bring slides to a boil in 10 mM sodium citrate buffer
pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool
slides on bench top for 30 minutes.
The problem is that bone detachment always occurs so I wonder if there is a
method of retrieval that prevents bone detachment.
Waiting to hear from you soon.
Thank you for your consideration
Best wishes
Hatem Salim