[Histonet] Cresyl Violet Counterstain of LFB
Patten, Nicole (NIH/NIAAA) [F]
pattennj <@t> mail.nih.gov
Thu Jun 4 08:54:58 CDT 2009
Hi Histonet... Quick question!!
I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this?
Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process?
Thanks in advance!
Nicole J. Patten
NIAAA/National Institutes of Health
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