[Histonet] Cresyl Violet Counterstain of LFB

[Patten, Nicole (NIH/NIAAA) [F]]

Hi Histonet... Quick question!!

I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this? 

Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process?

Thanks in advance!

Nicole J. Patten
Post-Baccalaureate Fellow/IRTA
NIAAA/National Institutes of Health