[Histonet] Please Help!

[Bonner, Janet]

I do several skin frozens every week and I know exactly how frustrating it can be when they roll!  However, the technique I use is simple : 
   As the edge of the skin section that is rolling is about to make contact with the knife/blade, put your soft brush bristles on top of the section and keep them on the section as you cut and the section rolls off the chuck.  If you're lucky the section will stay down on the plate and you can gently use the brush to tease the edges flat.  Then, you need to be fast and get it on the slide.   Once in a while I'll get a case that needs a fresh area of the blade after trimming to give me a decent section with the technique.  
   I hope this works for you.
         Have a great weekend!
                     Janet
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of DELIA GARCIA
Sent: Fri 7/31/2009 2:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Please Help!



Hello,

I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much.

Delia



     
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