[Histonet] IHC free floating sections

[Michael Patrick]

I would like to stain free floating sections (fixed rat brain; 50um). Is it necessary to transfer sections to "clean" culture wells with a paint brush every time I wash or incubate with antibodies? I find this causes significant damage to my tissue (especially since I do a lot of washes etc.). Could I simply use a pipette to draw out the old solution and then replace with the appropriate solution? What do most people do in these situations?

Much thanks

Mike

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