[Histonet] flash frozen tissue

[Kalleberg, Kristopher]

Hello All,
 
I will be running a study upcoming and we have decided not to run FFPE
as we normally do and have decided to use flash frozen.  My big concern
is what is the proper way to fix all of these SKIN samples to use for
IHC.  Any recommendations as to if prefix the samples in sucrose and
then flash freeze in OCT is best or any recommendations as to if post
fixing (in acetone??) the already sectioned, flash frozen sample in OCT
is best.  Any and and all help will be greatly appreciated.  Thank you.
 
Kris Kalleberg