[Histonet] Help with Connexin 43

[Amy Porter]

Anyone out there doing FFPE IHC on rat for Connexin 43 - I am having trouble getting the titration right on this & just wondering what types of dilutions anyone is using???  I am using a standard avidin/biotin complex staining system with and endogenous AV/BI block and not getting anything of any consequence in my negative control.  thanks - Amy

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu