[Histonet] double immunostaining with antigen retrieval and no antigen retrieval

[Cathy Malcontenti-Wilson]

Dear All,

Is it possible to do double immunostaining for one antibody which
doesn't require antigen retrieval, and the other one which doesn't need
it.  Can you do the one without retrieval first, then retrieve and do
the other one, or does the retrieval damage the first staining? We know
that doing antigen retrieval for antibody that doesn't require it gives
us non specific staining. 

 

I would appreciate some advice.

 

Thank you very much.
Cathy Malcontenti-Wilson