[Histonet] Vacuum embedding.

[Bryan Hewlett]

Hi Ian,

For paraffin wax infiltration, the normally quoted range of vacuum pressures 
to use varies between 360 mmHg(48 kPa) and 260 mmHg(34.66 kPa).
I always reduced the pressure slowly in the first wax to just 360 mmHg to 
prevent violent 'bumping' due to rapid outgassing of the clearant.
Second wax can be taken to 300 mmHg and third & fourth waxes to 260 mmHg.

Cheers,

Bryan


----- Original Message ----- 
From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, July 16, 2009 10:41 AM
Subject: [Histonet] Vacuum embedding.


>            We have recently bought a new vacuum embedding oven but there 
> is
> some dispute in the lab as to the correct vacuum pressure we should be
> using. I won't prejudice the replies by giving you our values, but what 
> are
> the common vacuum pressures that are being used?
>
> Ian.
>
>
>
>
>
> Dr. Ian Montgomery,
>
> Histotechnology,
>
> I.B.L.S. Support Unit,
>
> Thomson Building,
>
> University of Glasgow,
>
> Glasgow,
>
> G12 8QQ.
>
>
>
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