[Histonet] Re: Eosin in alcohol

[Mark Tarango]

I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.

Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.  Eosin washes out.

Mark Tarango HT(ASCP)QIHC

On Wed, Jul 1, 2009 at 10:06 AM, Jennifer Johnson
<jmjohnson34 <@t> hotmail.com>wrote:

>
> We have used Eosin in the last 95% alcohol on the tissue processor for
> several years.  I usually add approximately 5 ml to the full jug.  It is a
> great tool to use for embedding.  However, we received a letter from the lab
> that we send our prostate biopsies to saying that it was undesirable because
> it interfered with their immuno staining.  They sent us some cobalt blue to
> use in the place of eosin along with mixing instructions and the whole batch
> of tissues came out such a dark blue.  There is no delineations in the color
> of the blue and I found it to be useless for helping to embed.  I would
> rather do without anything than use cobalt blue.  I guess the point of my
> rambling is, Eosin is a wonderful tool to use unless you are doing immunos
> on prostate biopsies.
>
> Thanks,
>
> Jennifer Johnson, HTL (ASCP)
>
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