[Histonet] Green counterstain Issues

[Rene J Buesa]

That your tissue was fixed outside, but not inside, meaning, poor penetration most likely because of less than adequate time in the fixative. Increase the fixing time and most likely your staining unevenness will disappear.
René J.

--- On Tue, 1/27/09, Breeden, Sara <sbreeden <@t> nmda.nmsu.edu> wrote:

From: Breeden, Sara <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Green counterstain Issues
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, January 27, 2009, 2:26 PM

My (working) light green counterstain (Sheehan, Theory & Practice..., pg
187) does its thing unevenly.  Given, a lot of our tissue is poorly
fixed to begin with or possibly autolytic, but I get more staining
around the periphery and little or no staining in the body of the
section.  What am I missing here?  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

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