[Histonet] Green counterstain Issues

[Breeden, Sara]

My (working) light green counterstain (Sheehan, Theory & Practice..., pg
187) does its thing unevenly.  Given, a lot of our tissue is poorly
fixed to begin with or possibly autolytic, but I get more staining
around the periphery and little or no staining in the body of the
section.  What am I missing here?  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576