[Histonet] double staining on the ventana XT

[Piero Nelva]

We also run a p63/HMCK/p504s dual stain cocktail on prostate cores.  Easy to 
set up and results look great.

THe Kappa/Lambda dual stain works, too.  I find the red chromagen (lambda) 
in this setting is a little less precise, tho'.

REgards

Piero Nelva
Anatomical PAthology
Monash Medical Centre
----- Original Message ----- 
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
To: <histoinfo <@t> comcast.net>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 23, 2009 3:56 AM
Subject: AW: [Histonet] double staining on the ventana XT


Hi Jennifer,
we perform the doublestain routinly. The preferred protocol is CK HMW/p504s
on prostata biopsies.
The protocol is easy to establish and the staining results are very good.
The run lasts about 4 hours and we do it overnight. You need the Red and the
DAB ultraview kit. No extra reagens.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
histoinfo <@t> comcast.net
Gesendet: Donnerstag, 22. Jänner 2009 01:41
An: Histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] double staining on the ventana XT


Greetings,
We are looking into doing some immuno double staining. I searched the
archives and found a few similar inquiries but no responses. Anyone with
experiences to share I would love to hear what you have to say. Everything
from what not to do to what works well for you.
Many Thanks,
Jennifer



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


--------------------------------------------------------------------------------



No virus found in this incoming message.
Checked by AVG - http://www.avg.com
Version: 8.0.176 / Virus Database: 270.10.12/1910 - Release Date: 1/22/2009 
6:28 PM