AW: [Histonet] Starfrost slides

[Gudrun Lang]

One time we tried FISH on a cellsuspension dropped on a superfrost slide. It
was airdried. In the fluorescence microscope the nuclei were surrounded with
strong fluorescence. In another trial we heated the slides before FISH and
the slide was more or less clear.
I concluded, that the reagens on the slide surface must be altered through
the heat.
Just a single experience.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Nancy
Lemke
Gesendet: Donnerstag, 22. Jänner 2009 20:56
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Starfrost slides

Has anyone using Starfrost slides (7255) from Mercedes had any problems with
autofluoresence when doing IF?
Thanks in advance.
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit


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