[Histonet] Auto-IHC Staining Issues

[Rene J Buesa]

Not much help can you get if you do not say which autostainer you are having the problems with, because not all work in the same way.
René J.

--- On Wed, 1/21/09, Paula Lucas <plucas <@t> biopath.org> wrote:

From: Paula Lucas <plucas <@t> biopath.org>
Subject: [Histonet] Auto-IHC Staining Issues
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, January 21, 2009, 7:04 PM

Hello,

I've been having a problem with the staining quality from an auto-IHC
stainer and I was wondering if any of you experience the same thing.

I notice sometimes that the staining in the tissue isn't complete, like
there are areas where the fluid (one or more of the steps involved) didn't
get in contact with the tissue, thus resulting in patchy, skipped staining.  

I'm thinking that this must be a dispensing problem.

Is this a general thing with all auto stainers - ones like DAKO or Lab
Vision's stainer or Biocare Medical's stainer?  These stainers dispense
fluid onto the slide. 

I hope this explains things and I really hope that I can get some feedback. 
It's getting very frustrating.

Thanks in advance,
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA

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