[Histonet] texas red

[Bill McManus]

Eric:

Texas Red will interfere with the Alexi Flour488.  Methylene blue  
would work better.

Bill
On Jan 16, 2009, at 8:12 AM, ejschmid <@t> ucalgary.ca wrote:

> I need my GMA plastic sections to fluoresce, that is the plastic  
> itself.
> For the embedded tissue itself, I am using DAPI and Alexa Fluor 488.  
> So
> the stain I choose for the plastic, which I need to see using
> fluorescence, cannot interfere with the DAPI or Alexa Fluor 488.
>
> This is basically a triple labeling problem. The third stain I am
> considering is Texas Red. The reason is that I can get it without it
> conjugated to something (like a secondary) that makes it even more
> expensive. I wouldn't use it to stain cells or tissues, but rather the
> plastic of the thin sections (I know that sounds weird but my needs
> require a little thinking outside the box).
>
> So my basic question about whether Texas Red is a good choice breaks  
> down
> into two more specific questions: Will it work with the ALexa Fluor  
> 488?
> Is there another choice for a third stain that anyone can think of  
> that
> might be more suitable?
>
> Thanks!
> Eric
> University of Calgary
>
>
>
>
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Bill McManus
Western Dairy Center
Utah State University
bill.mcmanus <@t> usu.edu