[Histonet] Spit and Diastase

[Amos Brooks]

Yeah,
     Spitting on a slide is groody ... get a pig to do it instead hehe!
Amos


Message: 4
Date: Tue, 13 Jan 2009 13:16:10 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Spit and Diastase
To: histonet <@t> lists.utsouthwestern.edu, "Parker, Helayne"
       <HParker <@t> Skaggs.Net>
Message-ID: <168182.60463.qm <@t> web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Spitting to do a PAS "with diastase" is repugnant and unhealthy. Also it may
contaminate the sections with mouth bacteria.
Use a pig diastase, that is not as "readily available as the saliva you
carry around with you" but for certain more becoming.
Are you going to write in your SOP that s/he who is doing this procedure is
going to spit over the section? How much saliva? Before or after eating?
It is just revolting and disgusting!
René J.