[Histonet] Undecalcified bone in MMA and antigen retrieval

[gayle callis]

Neil Hand cut very thin sections, 0.5 to 1 um thin sections with a glass
knife or diamond knife.   The thickness very well may have made a difference
for section staying on the slide during the pressure cooker retrieval he
used (J Histotechnology, 21:231-236, 1998).   There are publications in J
Histotechnology by Nancy Troiano, et al where they microtomed PMMA embedded
bone sections - I do not recall what kind of slides or adhesives they used.
She has responses to Histonet questions and a search of the archives might
turn up her section adhesion methods.  Hopefully she is looking in to
provide comments. 

 

Good luck

 

Gayle M. Callis

HTL(ASCP)HT,MT

Bozeman MT