[Histonet] Histogel

[Bob Nienhuis]

Has anyone tried embedding a block of tissue like a mouse brain in Histogel,
and then cutting frozen or on a vibratome?

I am doing Golgi stain on mouse brain, and the tissue is VERY friable.

Website says, "No matter how small, friable, or viscous your histology
or cytology specimen, HistoGel will encapsulate and retain the entire
specimen during histological processing"

Bob Nienhuis
UCLA / VA Medical Center




On Tue, Jan 13, 2009 at 9:49 AM, Bartlett, Jeanine (CDC/CCID/NCZVED) <
jqb7 <@t> cdc.gov> wrote:

> Histogel works great and does not absorb water like plain agar does.
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
> Paul
> Sent: Tuesday, January 13, 2009 12:46 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Processing 3-dimensional cell cultures into
> paraffin
>
> I have processed and embedded cells grown in/on agar, or post-embedded
> in agar or agarose, many times.  It really isn't much different from
> processing and embedding tissue. You can trim the agar blocks to
> appropriate size with a scalpel (if necessary) either before or after
> fixation (if you trim them before fixation, do it while they are cold,
> so the agar is firm), and place them in a cassette just like a piece of
> tissue. Put them on the processor as usual, processing times the same as
> you would use for tissues of equivalent size, and embed as usual. One
> difference in sectioning however.  Don't place the blocks in water after
> facing them off.  The agar absorbs too much water and becomes soft.  The
> blocks should be cut cold, but not moistened.  Either place them on a
> cold plate without water, or put them in the refrigerator after facing,
> and take them out one at a time as you section them.
>
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