Re: Re: [Histonet] Fixation of the damaged brain tissue

[TF]

4% PFA
so u mean I should expose more of the damaged site (it is at the bottom of the brain, or olfactory bulb)
and immerse the whole brain in a larger volume of fixatives?

I think PFA penetrate quite fast, and I took all the skins & muscles off the skull before put the who brain with skull into PFA for 1-2 days.
It should work...

The perfusion is not well in many conditions - in my injury model, part of the blood supply was interrupted.


2009-01-12 



TF 



发件人： Geoff McAuliffe 
发送时间： 2009-01-12  22:59:22 
收件人： tifei 
抄送： histonet 
主题： Re: [Histonet] Fixation of the damaged brain tissue 
 
Hi TF:
Your problem should not be happening.
Sounds like poor perfusion followed by immersing the whole brain (with 
the wound deep in the interior) in a small volume of fix at 4 degrees C.
What fixative are you using?
Geoff
TF wrote:
> Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury).
> Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site...
> I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot.
> Can anyone provide some suggestions?
>
> 2009-01-12 
>
>
>
> TF 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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