[Histonet] Fixation of the damaged brain tissue

[Geoff McAuliffe]

Hi TF:

Your problem should not be happening.
Sounds like poor perfusion followed by immersing the whole brain (with 
the wound deep in the interior) in a small volume of fix at 4 degrees C.
What fixative are you using?

Geoff

TF wrote:
> Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the injury).
> Because the injury damaged the surrounding blood supply, and after perfusion I normally got a mass of soft, shapeless tissue surrounding the injury site...
> I have also tried post-fixation of 24-48 hours before removing the brain from in situ. But it does not help a lot.
> Can anyone provide some suggestions?
>
> 2009-01-12 
>
>
>
> TF 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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