[Histonet] Cryostat safety question

[Jan.Minshew <@t> leica-microsystems.com]

Hi Andrea,

It's great to hear that you are happy with your cryostat and, hopefully,
I'll be able to help you with an answer to your question.  Leica sells a
small device called a Thermal Block that is designed to help you remove the
specimen from the chuck without causing it to melt.  The catalog number is
14039818542.  It's a small, mobile device that can be used in any of our
cryostats that use chucks with stems in the back (we have a different one
for the new CM1950 flat backed chucks).
If you have questions or would like additional information, please feel
free to contact our Technical Applications Center (1-800-248-0123 option 1
then option 2).

Best wishes,


Jan Minshew, HT/HTL(ASCP)
Marketing Manager
Leica Microsystems
Biosystems Division
2345 Waukegan Road
Bannockburn, IL 60015

800.248.0123 Toll Free
847.405.7051 Direct
847.405.6560 Fax

www.leica-microsystems.com

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             "Andrea Hooper"                                               
             <anh2006 <@t> med.corn                                             
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             01/07/2009 05:40          [Histonet] Cryostat safety question 
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The discussion on microtome safety begs me to ask a cryostat question ....

We have a Leica CM3050 cryostat and love it!

How are people (and perhaps only those in research do this) removing
their tissue from the chucks for future use? We often just section a
few slides worth then put the block at -80 deg C for future studies.
Needless to say, it's the most dangerous part of our day.

So what are your suggestions for removing tissue from a chuck (and
melting it isn't really a viable option)?

Thanks in advance,
Andrea
--

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